m orale (ATCC)
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m orale
M Orale, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m+orale/Genomic+DNA+from+Mycoplasma+orale+strain+CH+19299/pm37878143-78-34-36
Average 92 stars, based on 1 article reviews
M Orale, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m+orale/Genomic+DNA+from+Mycoplasma+orale+strain+CH+19299/pm37878143-78-34-36
Average 92 stars, based on 1 article reviews
m orale - by Bioz Stars,
2026-09
92/100 stars
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Biomarker Discovery:Article Title: A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasma pneumoniae based on MALDI-TOF MS Article Snippet: .. Twenty other pathogens, including M. genitalium (ATCC 33530), M. salivarium (ATCC 23064), other:Article Title: Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification. Article Snippet: Mycoplasma contamination in cell culture affects the properties of cell lines.. Gold standard detection by microbiological culture takes days and requires specialists.. The polymerase chain reaction and loop-mediated isothermal amplification (LAMP) are fast molecular options, but LAMP only requires one heating block for DNA amplification. Article Title: In situ immunohistochemical detection of intracellular Mycoplasma salivarium in the epithelial cells of oral leukoplakia Article Snippet: Article Title: Impact of Aerobicity on Mollicute Colony Growth on Agar Article Snippet: Mycoplasma detection assays employing the direct culture (broth and agar) approach are described in various regulations and compendia (e.g., 21 CFR 610.30 [1], United States Pharmacopeia <63> [2], United States FDA 1993 Points to Consider [3], and European Pharmacopoeia 2.6.7 [4]).. The earliest approach (21 CFR 610.30) specified incubation under both aerobic and anaerobic conditions, while the other approaches specified incubation under low oxygen (microaerophilic) conditions only.. The decision as early as 1993 to use only microaerophilic conditions was based on empirical studies conducted by the FDA demonstrating that mollicute (e.g., mycoplasma, acholeplasma, spiroplasma, ureaplasma) growth in broth and agar under such conditions was approximately equivalent to aerobic and therefore no significant advantage was conferred by using both incubation conditions [5]. Polymerase Chain Reaction:Article Title: Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran Article Snippet: Accordingly, Nutrient Broth (Sigma-Aldrich ® , Cat No: 70122 FLUKA), Sabouraud Dextrose Broth (BD-Difco, Cat No: 238230, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBLTM, Cat No: 297642), Brain Heart Infusion Broth (BD-BBLTM, Cat No: 221812), Trypticase Soy Broth (BD-BBLTM, Cat No:221715), Yeast Malt Broth (Sigma-Aldrich ® , Cat No: Y3752), Blood Agar (Sigma-Aldrich ® , Cat No: 70133 FLUKA), Nutrient Agar (BD-DifcoTM, Cat No: 212000), MacConkey Agar (Sigma-Aldrich ® , Cat No: 70143 FLUKA), Sabouraud Dextrose Agar (BD-RODACTM, Cat No: 295872), and Brain Heart Infusion agar (BD-BBLTM, Cat No: 211065) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi 2005 ). table ft1 table-wrap mode=article t1 Table 1 caption a4 List of selected cell lines for checking of mycoplasma contamination in the present study Positive and negative controls for PCR technique The DNA of different mollicutes and bacterial strains were supplied from American Type Culture Collection (ATCC, Manassas, VA, USA) and National Collection of Type Cultures (NCTC, Salisbury, UK). .. Ureaplasma urealiticum (NCTC 10177T), M. genitalium (NCTC 10195), M. salivarium (NCTC 10113), M. pneumonia (NCTC 10119), A. laidlawii (ATCC 23206), Bacteria:Article Title: Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran Article Snippet: Accordingly, Nutrient Broth (Sigma-Aldrich ® , Cat No: 70122 FLUKA), Sabouraud Dextrose Broth (BD-Difco, Cat No: 238230, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBLTM, Cat No: 297642), Brain Heart Infusion Broth (BD-BBLTM, Cat No: 221812), Trypticase Soy Broth (BD-BBLTM, Cat No:221715), Yeast Malt Broth (Sigma-Aldrich ® , Cat No: Y3752), Blood Agar (Sigma-Aldrich ® , Cat No: 70133 FLUKA), Nutrient Agar (BD-DifcoTM, Cat No: 212000), MacConkey Agar (Sigma-Aldrich ® , Cat No: 70143 FLUKA), Sabouraud Dextrose Agar (BD-RODACTM, Cat No: 295872), and Brain Heart Infusion agar (BD-BBLTM, Cat No: 211065) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi 2005 ). table ft1 table-wrap mode=article t1 Table 1 caption a4 List of selected cell lines for checking of mycoplasma contamination in the present study Positive and negative controls for PCR technique The DNA of different mollicutes and bacterial strains were supplied from American Type Culture Collection (ATCC, Manassas, VA, USA) and National Collection of Type Cultures (NCTC, Salisbury, UK). .. Ureaplasma urealiticum (NCTC 10177T), M. genitalium (NCTC 10195), M. salivarium (NCTC 10113), M. pneumonia (NCTC 10119), A. laidlawii (ATCC 23206), Modification:Article Title: Flow-Cytometric Method for Viability Analysis of Mycoplasma gallisepticum and Other Cell-Culture-Contaminant Mollicutes. Article Snippet: Mycoplasma is the smallest self-replicating bacteria, figuring as common contaminant of eukaryotic cell cultures.. Production inputs and operator’s manipulation seem to be the main sources of such contamination.. Many analytical approaches have been applied for mycoplasma detection in cell cultures and also in biological products. |